Robert Reagan Holloway
Curriculum
Vitae
Education
Ph.D. 1976
University of Texas at Austin, Austin, TX Physical
Biochemistry
M. A. 1972
Rice University, Houston, TX Physical Organic Chemistry
B. A. 1968
Rice University, Houston, TX Chemistry and Mathematics
Industrial Chemistry,
from Research Lab to Marketplace,
invited speaker, March 20, 2003, Trinity University, San Antonio, TX
Carboxyl Terminal Amino
Acid Visualization, poster with
Marcus J. Horn, February 20-24 2000, ABRF 2000 Conference, Bellevue, Washington
Visualization of Amino
Acids in Carboxyl Terminal Protein Sequencing,
poster with Marcus J. Horn, February 3-10
2000, Lorne Protein Conference, Lorne, Australia
Examination of
Carboxypeptidase-Like C-Terminal Sequencing,
poster with Marcus J. Horn, July 24-28, 1999, !3th Conference of the Protein
Society, Boston, MA.
Two-Dimensional
Separations Using On-Line Liquid Chromatography-Capillary Electrophoresis, invited speaker with coauthors Wesley Cole,
Catherine Keely-Templin, Vonda Smith, and Tom
Van de Goor, January 25, 1996, HPCE 96, Orlando, FL.
Tandem LC-CE Analysis by
Capillary Electrophoresis,
poster with Vonda K. Smith, Catherine Keely-Templin, and Wesley D. Cole,
October 23-25, 1995, The 6th Frederick Conference on Capillary
Electrophoresis, Frederick, MD.
Capillary
Electrophoresis: A Product of
Technological Fusion, Hewlett-Packard Journal, 46(1995), 6-9.
Radial Field Sensitive
Electroosmotic Flow in Nonsilica CE Systems, poster
with Wesley D. Cole, Catherine A. Keely, Tom A. van de Goor, and James E.Young,
January 31-February 3, 1994, HPCE 94, San Diego, CA.
Flow Profile in
Capillary Electrophoresis Due to External Electric Fields, speaker Tom A. A. M. van de Goor, coauthors
Catherine A. Keely and Douglass McManigill, February 1, 1994, HPCE 94, San
Diego, CA.
Dispersion in Capillary
Electrophoresis with External Flow Control Methods, article and talk with speaker Catherine A. Keely,
coauthors Tom A. A. M. van de Goor, and Douglass McManigill, Journal of
Chromatography A, 652(1993) 283-289, January 26, 1993, HPCE 93, Orlando FL.
A Noncrosslinked
Organosilicon Matrix for Luminescence Quenching Based Fiber Optic Sensors, article and invited talk with speaker Ganapati
Mauze, January, 1993, SPIE Conference on Progress in Biomedical Optics, Los
Angeles, CA.
Enhancement of
Performance in Free Solution Capillary Electrophoresis by Bulk Flow Control, poster with Cathy Keely, Doug McManigill, and Jim
Young, January 25-28, 1993, HPCE93, Orlando, FL.
Applications of the
Control of Electroosmotic Flow in Capillary Electrophoresis, invited speaker, with coauthors Juergen A. Lux,
Cathy Keely, and Jim Young, March 12, 1992, Pittsburgh Conference 1992, New
Orleans, LA.
Resolution Optimization
in Capillary Electrophoresis by Electronic Adjustment of Electroosmotic Flow, poster with J. A. Lux, C. A. Keely, D. McManigill,
and J. E. Young, February 11, 1992, HPCE 92, Amsterdam, The Netherlands.
Methods of
Polyacrylamide Polymerization for Capillary Gel Electrophoresis, speaker D. J. Rose, Jr., March 18, 1991,
International Meeting of the Electrophoresis Society, Washington, D.C.
Methods of
Polyacrylamide Polymerization for Capillary Gel Electrophoresis, speaker D. J. Rose, Jr., February 5, 1991, HPCE
91, San Diego, CA.
Prepolymerization of
Acrylamide in the Fabrication of Wall Bound Gel Filled Capillaries for
Electrophoresis, poster with Donald J. Rose, February 4, 1991,
HPCE 91, San Diego, CA.
Selectivity in Capillary
Gel Electrophoresis, speaker D.
J. Rose, March 5-9, 1990, Pittsburgh Conference 1992, New York, NY
Studies in the
Preparation of Columns for Polyacrylamide Gel Capillary Electrophoresis (PAGCE), poster with Donald J. Rose, January 30, 1990,
HPCE 90, San Francisco, CA.
Bidentate Ligand
Substitution Reactions on Ruthenous Complexes, talk,
coauthors Teddy Kiang and Stuart Berryhill, December 21, 1984, International
Chemical Congress of Pacific Basin Societies, Honolulu, HI.
pH Sensitive Dyes as
Environmental Probes in Hydrophilic Matrices,
talk, coauthors Charles R. Connell and Teddy Kiang, March 20, 1983, American
Chemical Society National Meeting, Seattle, WA
Immobilization of
Phenolsulfonthalein on Controlled Pore Glass in a New Biochemical Sensor, Chemistry Department Seminar, Long Beach State
University, March 31, 1982, Long Beach, CA
MO Calculations of
Oxalate and Related Systems, poster
with Andrew Streitwieser, March 14, 1978, American Chemical Society National
Meeting, Anaheim, CA
Is Aromaticity Absolute?, with Nathan Bauld, Terry Welsher, and James
Cessac, Journal of the American Chemical Society, 100(1978),6920.
1,3 (nonbonded)
Carbon/Carbon Interactions: The
Common Cause of Ring Strain, Puckering, and Inward Methylene Rocking in
Cyclobutane and of Outward Methylene Rocking in Cyclobutyl Cation,
with Nathan Bauld and James Cessac, Journal of the American Chemical Society,
99(1977), 8140.
The Cyclobutene/Butadiene
Anion Radical Electrocyclic Reaction,
with Nathan Bauld, James Cessac, C. Chang and F. R. Farr, Journal of the
American Chemical Society, 98(1976), 4561.
Computer Simulation of
Sedimentation in the Ultracentrifuge. VIII.
Solutes Undergoing Indefinite Association, with David Cox, Archives of Biochemistry and
Biophysics, 160(1974), 595.
I. Sedimentation Velocity Studies of Molpadia Arenicola
Hemoglobin and Escherichia Coli Pyruvate Dehydrogenase
II. Computer Simulation of
the Sedimentation of Associating Systems,
Ph.D. Dissertation, May 1976, University of Texas at Austin, Austin, TX
I. The Gas Phase Rearrangement of Crotyl Trifluoroacetate in an
Artificially Stirred Flow Reactor. II.
The Rearrangement of Methy Allyl Xanthate, Master’s
Thesis, 1972, Rice University, Houston, TX
Monolithic Silica
Column, US 6210570 (Apr 3, 2001).
Preparative Capillary
Electrophoresis with Wide Bore Capillaries, with
H. F. Yin, C. Templin, D. McManigill, US
5658446 (August 19, 1997)
Capillary
Electrophoresis Flow Control System, with
C. Keely-Templin, D. McManigill, US 5482608 (January 9, 1996)
Capillary
Electrophoresis Using Zwitterion-coated Capillaries,
US 5415747 (May 16, 1995)
Chemically Sensitive,
Dimensionally-stable Organosilicon Material Composition and Techniques, with G. Mauze, D. Spira-Solomon, US 5194391 (Mar
16, 1993).
Capillary Gel
Electrophoresis Columns and Method of Preparing the Same,
US 5167783 (Dec 1, 1992).
Capillary Gel Column
with Polyorganosiloxane Coupling Layer,
US 5110439 (May 5, 1992).
Covalently Labelled
Siloxane Polymers, with T.
Kiang, US 5070158 (Dec 3,1991).
Chemically Sensitive,
Dimensionally-stable Organosilicon Material Composition, with G. Mauze, D. Spira-Solomon, US 5057277 (Oct
15, 1991).
November
2001 to August 2002
Marketing
Consultant, Contractor
Acros Organics, New Jersey.
Developed marketing data for organic and biochemical sales initiative.
Represented Acros on site at Exelixis Corporation, South San Francisco,
CA
Spring
1988 to early 1992:
Seminar Coordinator for Analytical/Medical Laboratory Hewlett Packard Labs Medical/Analytical Laboratory. Set up visits and lectures at HP Labs by distinguished scholars, scientists, technologists and figures of notable interest, including Carl Djerassi, synthetic chemist, entrepreneur and novelist, and James Lovelock, originator of the Gaia hypothesis.
Fall
1980-Spring 1987 semesters:
Instructor at West Valley College, Mission College, and Skyline College. Organic chemistry, general chemistry, and analytical chemistry. Specialized in non-major general chemistry at West Valley, later conducted organic and analytical courses.
1969-72:
Quantitative and qualitative laboratory teaching, tutoring, Rice University.
1972-75:
Organic chemistry, biochemistry laboratory teaching and tutoring, University of Texas.
University Teaching
August
2003 to present
Assistant
Professor of Chemistry Schreiner University.
Biochemistry, General Chemistry, Physical Chemistry, Science Teaching
1976:
Substitute Lecturer for
Professor Nathan Bauld in Organic Chemistry, University of Texas.
1998-2002:
Instructor University of California Berkeley Extension. Biochemistry, Organic Chemistry
Postdoctoral Work
December
1976 to July 1978:
Researcher University of
California at Berkeley under Professor Andrew
Streitwieser. Worked in hydrocarbon
acidity. Synthesis of
phenylbenzanthrene. .
Ab initio calculations on lithium oxalate.
January
1976 to December 1976:
University of Texas at Austin under Professor Nathan Bauld. Welch Postdoctoral Fellowship. Ab initio calculations on radical cations.
January
1972 to December 1976:
German Translator and Technical Editor, Ralph McElroy Co., Austin, TX. Edited English translation of Kobunshi Ronbunshu, Japanese polymer and textile chemistry journal.
February
1999 to August 2001
Senior
Research Chemist in protein chemistry.
Biomolecular Technologies, Inc.
Sunnyvale, CA. Methods development, automation development (reactions,
liquid transfer, concentration by evaporation),
fluorescent labeling of amino acids, for novel carboxyl terminal
protein sequencing chemistry. Helped
develop peptide capture tools (PI3™), manufacture them, and
market them directly to potential customers..
Used solid phase
extraction methodology (C18 minicolumns)
extensively for isolation of peptides and proteins.
Performed analytical
instrument maintenance and repair, especially HPLC.
January
1998 to February 1999:
Member
of the Technical Staff, Hewlett Packard Labs
Medical Department, Palo Alto,CA. Assessment
of Point of Care Testing technology for possible HP entry into field.
Responsible as chemistry/biochemistry expert on blood assays
anticipated in a medical measurement device designed for low cost,
connectivity and portability.
April 1997 to December 1997:
Member of the Technical Staff, Hewlett
Packard Labs Medical Department, Palo Alto,CA.
Investigation for opportunities in the Drug
Discovery industry. Worked as
a partner with HP division in evaluating alternative approaches for the
comporation’s analytical group, studying the state of the art in
combinatorial chemistry and high throughput screening.
Began a project to develop a fluorescence screening system for highly
parallel bioassays, synthesized new fluorescent dyes.
Initiated project in electrochemiluminescence.
Surveyed all major entities in drug discovery instrumentation business,
including vertically integrated pharmaceutical companies.
November
1996 to March 1997:
Member of the Technical Staff, Hewlett
Packard Labs Analytical Department, Palo Alto,CA.
Monolithic Column Research.
Formation of silicas from organic silanes for use in
chromatography. Patent
sought for novel method producing in
situ gel with no syneresis.
July
1994 to October 1996:
Member of the Technical Staff, Hewlett
Packard Labs Analytical Department, Palo Alto,CA.
Worked on integration of liquid chromatography and capillary electrophoresis.
Investigated 2D LC-CE as practiced by Jim Jorgenson of UNC.
Analyzed tryptic digests of recombinant human growth hormone and tissue
plasminogen activator. Presentation
to international fora in HP's
unique implementation of technique.
April
1988 to July 1994:
Member of the Technical Staff, Hewlett
Packard Labs Analytical Department, Palo Alto,CA.
Worked in the area of capillary electrophoresis. Developed
polyacrylamide based hydrophilic gel support matrices with far greater
lifetime than existing gels. These
materials must withstand repeated passage of chemically aggressive media (SDS,
etc) under high electric field strengths (1000 volts per centimeter).Worked on
development of electronic control of electroosmotic flow.
Patent granted for novel method of suspending gel filling from
capillary wall by a compliant chemical link (US 5,110,439), another for
prepolymer filling, with crosslinking, wall linkage in situ (US
5,167,783). Patent granted for
novel hydrophilic interior coating for CE capillaries (US 5,415,747). Patent
granted in the area of hydrodynamic flow control in capillary electrophoresis
(US 5,482,608).
Developed
techniques for the production of CE columns of capacity high enough for MALDI
sample preparation. (US 5658446)
1980
through March 1988:
Member of the Technical Staff, Hewlett
Packard Labs Medical Department, Palo Alto, CA.
Worked on all phases of development of a blood
gas sensing system (medical application).
Fiber optic system had no electronic parts in contact with human body,
so all analytes sensed by optical transduction of chemical response;
worked to satisfy requirements of containment, stability, sensitivity,
specificity, immunity to interferences, sterilization, rheology of packing
material, toxicity studies, biocompatibility, all chemical aspects.
Handled many analytical needs, including GC/MS analysis, NMR, etc.
Worked also on instrumentation, sensor fabrication, testing, market
targeting and customer feature expectations. Three patented inventions in
course of work (US 5,194,391, US 5,070,158 and 5,057,277).
One involved attachment of ruthenous tris-diimide dyes to silicone
polymers via a trifunctional crosslinker;
the novel aspect was the choice of acetic anhydride/acetic acid as a
solvent system during replacement of the acetate ligand on the methyl
triacetoxysilane crosslinker, a reaction which required a polar solvent but
was very sensitive to reaction with solvent.
The other two are a silicone grease with a dye imbedded which is
suitable for incorporation into a fluorescence quenching based oxygen sensor
and a process for making that material. This
material makes novel use of silicone rubber technology.
Rather than a hydroxyl terminated polydimethylsiloxane, an inert
polydimethylsiloxane is used in the formulation, so that a cured rubber is
never formed; rather, the usual
silicone crosslinker here serves as a modifier to adjust the oxygen sensitive
linear range of the quenching interaction.
The rheology of the final material is more suitable for many
applications than a rubber.
Aug
1978 to early 1980:
Member
of the Technical Staff, Hewlett Packard Labs
Medical/Analytical Department, Palo Alto, CA.
Worked to select pH sensor
chemistry for planned bedside blood gas instrument.
Developed cuvette with pH sensitive wall for total internal reflection
sensing (increases path length)
.June 1968 to January 1969:
Pilot plant operation and trouble-shooting, caprolactam plant. Dow Badische Chemical Co., Freeport, TX.
Computer
Operator
May
1966 to January 1968:
Computer
operator.
Texaco, Inc. Bellaire, TX
Worked full-time while a full-time student at Rice University.
Graduate Work
1972-76:
Ph.D.,
University of Texas at Austin
Studied the dimerization of
Molpadia Arenicola hemoglobin by sedimentation velocity in the
ultracentrifuge. Invented a novel
pseudo finite difference simulation of rapidly dimerizing systems in the
ultracentrifuge and predicted conditions under which three bands would be seen
in the sedimentation velocity record. Under
Professors David Cox, Petr Munk.
1969-71:
M.A., Rice University Designed a mechanically stirred reactor for the study of gas phase organic reactions faster than the diffusion controlled rate. Under Professor E. S. Lewis
1967:
Undergraduate
research Technical help Rice University in the laboratory of Robert Curl [In
1996 Dr. Curl won the Nobel Prize in Chemistry for work unrelated to that done
in 1967]
Awards
2003:
Elmore Whitehurst Award for Creative Teaching, Schreiner
University
Personal
Family:
Wife and one daughter.
Pastimes:
Coastside
Chorale, trombone playing in Symphony of the Hills, Hewlett Packard Symphony,
Half Moon Bay Orchestra, and Dixie Downbeats;
Jogging, tennis.
Other Activities: Chairman, Board of Directors, San Mateo County American Diabetes Association, 1991-2. Secretary, Board of Directors 1992-3, Board Member 1989-93. Delegate to ADA California Affiliate, 1990, 1992.
Home
(830) 896-8138
Work
(830) 792-7250
Fax
(830) 792-7439
email
bholloway@schreiner.edu